Growth Inhibition of Struvite Crystals in the Presence of Herbal Extract Boerhaavia diffusa Linn

نویسنده

  • C. K. Chauhan
چکیده

Problem statement: The formation of a urinary stone, known as nephrolithiasis, urolithiasis, renal calculi or kidney stone is a serious, debilitating problem in all societies throughout the world. Struvite or Ammonium Magnesium Phosphate Hexahydrate (AMPH) is one of the components of urinary stone (calculi). Struvite stones are commonly found in women. Struvites form in humans as a result of urinary tract infection with ureolithic urea splitting micro organisms. These stones can grow rapidly forming "staghorn-calculi", which is more painful urological disorder. Therefore, it is of prime importance to study the growth and inhibition of Struvite crystals. Approach: This in vitro study had been carried out in the presence of herbal extract of Boerhaavia diffusa Linn. by using single diffusion gel growth technique. Sodium metasilicate solution of specific gravity 1.05 and an aqueous solution of ammonium dihydrogen phosphate of 0.5 M concentration were mixed so that the pH value 7.0 could be set. After the gelation, equal amount of supernatant solution of 1.0 M magnesium acetate prepared with 0.5 and 1% concentrations of the herbal extract of B. diffusa Linn. were gently poured on the set gels in the respective test tubes in the aseptic medium. Results: The growth of crystals without and with herbal extracts was monitored at regular time intervals. As the concentration of B. diffusa Linn. increased, the inhibition of crystals also increased in the gel media as well as the dissolution of crystals at the gel-liquid interface increases. The de-fragmentation of some grown crystals was also noticed. Conclusion: The herbal extract of B. diffusa Linn. inhibited the growth of struvite crystals in vitro. This study incorporated multidisciplinary interests and may be used for formulating the strategy for prevention or dissolution of urinary stones.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Inhibition of Human Cervical Cancer Cell Growth by Ethanolic Extract of Boerhaavia diffusa Linn. (Punarnava) Root

In Indian traditional medicine, Boerhaavia diffusa (punarnava) roots have been widely used for the treatment of dyspepsia, jaundice, enlargement of spleen, abdominal pain and as an anti-stress agent. Pharmacological evaluation of the crude ethanolic extract of B. diffusa roots has been shown to possess antiproliferative and immunomodulatory properties. The extract of B. diffusa was studied for ...

متن کامل

Chemotherapeutic potential of Boerhaavia diffusa Linn: A review

Article history: Received on: 13/12/2012 Revised on: 04/01/2013 Accepted on: 18/01/2013 Available online: 29/01/2012 Boerhaavia diffusa Linn. has been shown to exhibit a wide range of medicinal properties for the treatment of diabetes, inflammation, stress, hepatotoxicity, jaundice and heart failure. The extraordinary antioxidant, hepatoprotective, antibiotic, antidiabetic and anticarcinogenic ...

متن کامل

In Vitro Antioxidant and Cytotoxic Analysis of Boerhaavia diffusa Linn

The present study was carried out to evaluate the antioxidant and cytotoxic activity of unexploited plant, Boerhaavia diffusa indigenous to India. Different concentrations of the ethanolic whole-plant extracts (1000, 500, 250, 125, 50, 25, 12.5 μg/ml) were subjected to 1,1-diphenyl -2-picryl hydrazyl (DPPH) radical scavenging, reducing power activity and cytotoxic study against vero cell lines....

متن کامل

Antimicrobial activity study of ethanolic extract of Boerhaavia diffusa whole plant

The search for antimicrobial agents has continued to be concentrated on the lower plants, fungi and bacteria. Less research has focused on higher plants although identified plant compounds like emetine, berberine quinine still find specialized uses. Secondary metabolites of higher plants serve as defense agent against microorganisms. Some screenings have yielded good result and few of these hav...

متن کامل

Growth inhibition of struvite crystals by the aqueous root extract of Rotula aquatica.

Formation of urinary stone is a serious and debilitating problem throughout the world. In the present study, the inhibitory effect of aqueous extract of root of Rotula aquatica was investigated against struvite crystals (one of the components of urinary stone) grown in vitro using single diffusion gel growth technique. For setting the gel, sodium metasilicate solution (specific gravity 1.05) an...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2009